‘Cultured mast cells and basophils (1-5 × 10 5-6 cells/ml in Tyrode's solution) were stimulated with 5 to 20 g/ml of rSPE B / SCP, SPE A, SPE C or 1 g/ml of lipopolysaccharide.’
‘Cells were perfused at room temperature in a modified Tyrode's solution consisting of 150 mM NaCl, 5 mM KCl, 2 mM CaCl 2, 1 mM MgCl 2, 10 mM Hepes, 30 mM glucose.’
‘Hearts were perfused for 5-7 min with nominally Ca free Tyrode's solution, then perfusion proceeded with added collagenase and albumin.’
‘Hearts were excised quickly, placed on a Langendorff apparatus and perfused for 5 min with nominally Ca-free Tyrode's solution.’
‘The resulting cell suspension was centrifuged for 3-4 min at 2000 rpm and resuspended in a Tyrode's solution containing (in mM) 150 NaCl, 4 KCl, 2 CaCl 2, 2 MgCl 2, 10 glucose, 10 HEPES, pH adjusted to 7.4 with NaOH.’
Origin
1920s named after Maurice V. Tyrode (1878–1930), American pharmacologist.
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