Definition of elute in English:


transitive verb

[with object]Chemistry
  • Remove (an adsorbed substance) by washing with a solvent, especially in chromatography.

    ‘these protein bands were eluted and used as immunogen’
    • ‘After repeated washing captured proteins were eluted from the resin using 10 mM DTT.’
    • ‘Bound material was then eluted with 0.2 M methyl mannoside in the same buffer.’
    • ‘After extensive washing of the unbound protein with 40 mM imidazole, the GHS protein was eluted with 250 mM imidazole.’
    • ‘The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole.’
    • ‘A radioactive compound identified as p-coumaroyl ß-glucosyl ester was eluted and stored frozen.’



/əˈlo͞ot/ /əˈlut/


1920s from Latin elut- ‘washed out’, from the verb eluere, suggested by German eluieren.