Definition of ultracentrifuge in English:

ultracentrifuge

Pronunciation /ˌəltrəˈsentrəˌfyo͞oj/ /ˌəltrəˈsɛntrəˌfjudʒ/

noun

  • A very fast centrifuge used to precipitate large biological molecules from solution or separate them by their different rates of sedimentation.

    ‘After removing the cell debris by centrifugation, supernatant was layered on a 5.7 M CsCl / 0.01 M EDTA solution in an ultracentrifuge tube and centrifuged in a swinging bucket rotor at 40,000 rpm for 16 hr.’
    • ‘The supernatant was recentrifuged at 109,000 g on a tabletop ultracentrifuge (Rotor TLA 45 of Beckman centrifuges, Beckman Coulter, Fullerton, CA) for 1 h.’
    • ‘PLY solution species from monomer via multimeric intermediates to ring-shaped oligomers were studied with time-dependent sedimentation velocity in the analytical ultracentrifuge.’
    • ‘Boundary sedimentation and sedimentation equilibrium experiments were performed using an analytical ultracentrifuge Optima XL-I from Beckman Instruments (Palo Alto, CA) equipped with absorbance and interference optics.’
    • ‘The sarcosine insoluble fractions were removed by centrifugation at 100,000 g for 1 h in an ultracentrifuge, washed once with PBS, pH 7.4 and used as outer membrane protein fractions.’

transitive verb

[with object]
  • Subject to the action of an ultracentrifuge.

    ‘the samples were ultracentrifuged for two hours’
    • ‘The sample was then ultracentrifuged for 1 hour at 141,000 × g to remove membranes.’
    • ‘To concentrate the solution, the collagen was ultracentrifuged at 10 deg C for 26-48 It for preparation of 10-45 mg/ml gels.’
    • ‘An additional 34 ml buffer solution was added and the sample was then ultracentrifuged at 100,000 × # for 4 h.’
    • ‘The supernatant was ultracentrifuged at 105,000 × g, 4°C for 1.5 h.’
    • ‘The homogenate, obtained after sonication, was ultracentrifuged at 60 000 g for 30 min.’
    • ‘The remaining sample was ultracentrifuged to obtain the sputum sol phase.’